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Determination of Volatile Patty Acids in Bovine Blood by Isotope Dilution^{1, 2,}

Dairy Department, University of Maryland, College Park

ABSTRACT

The levels of acetate, butyrate, and valerate in venous cow blood were determined by an isotope dilution technique involving an acidified acetone-ethanol extraction procedure. It was found that acetate accounts for most of the total volatile fatty acids (VFA) in venous bovine blood and probably is present in greater amounts than heretofore reported. The isotope dilution technique is a valuable tool for the determination of the level of the VFA in blood, but maximum precision and accuracy probably will not be possible until the mode of transport of VFA in blood is understood.

¹ Scientific Article No. A722. Contribution No. 2958 of the Maryland Agricultural Experiment Station.

² Data presented in this paper are taken from a thesis submitted by J. B. Hotter in partial fulfillment of the requirements for the M.S. degree.