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Cold Shock of Epididymal Spermatozoa

Department of Dairy Husbandry, University of Wisconsin, Madison

ABSTRACT

Lasley and coworkers (3, 4, 5) reported that epididymal spermatozoa were resistant to cold shock and that ejaculated spermatozoa were not. They have likewise reported that ejaculated seminal plasma did not affect the cold shock response of epididymal spermatozoa and that as spermatozoa progressed from their place of origin outward they became more sensitive to shock. Bialy and Smith (1) reported that in the ductus deferens and ampulla of the bull there was not a sufficient number of spermatozoa to comprise an average ejaculate. Thus, it appeared that during their transition from the epididymis to the time that spermatozoa became a part of the ejaculate, the cells became susceptible to cold shock.

Spermatozoa for this study were obtained from slaughterhouse bulls. Testes and ampullae from the same bull were kept together. The time interval between the slaughter and the beginning of experimental work was approximately 2 hr. Spermatozoa from the cauda epididymis and the ampulla were washed, out under pressure with a 2.9% sodium citrate solution, utilizing about 15 ml. of fluid.

¹ Published with the approval of the Director of the Wisconsin Agricultural Experiment Station.

² Department of Dairy Science, University of Arizona, Tucson.