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Estrogenic Activity in Milk of Cows and the Bile of Calves Fed Low Levels of Diethylstilbestrol¹

Department of Zoology and Department of Dairy Husbandry Kansas State University, Manhattan

ABSTRACT

Milk from cows fed diethylstilbestrol (DES), and bile from calves fed this substance, were extracted and tested in castrate mice, using uterine weight as the criterion for measuring estrogenic activity.

The following conclusions were drawn:

Extracts of milk from untreated cows, in different phases of sexual cycles, gave no estrogenic response.

Extracts of milk from cows fed 10 mg. diethylstilbestrol (DES) per 1,000 lb. body weight per day caused no measurable estrogenic response.

Test mice consistently responded to known amounts of 0.048 mg. DES each.

Known amounts of DES were placed in milk, then extracted. These extracts caused consistent responses for estrogenic substances in test mice.

Bile from calves that had been given 10 mg. DES daily was extracted. This extract in test mice caused responses indicating that a daily collection of bile contained 46.08 µg. of free DES or its equivalent of estrogenic substance. Bile extracts from an untreated male calf indicated no estrogenic response.

¹ Contribution No. 291, Serial No. 332, Department of Zoology, and No. 272, Department of Dairy Husbandry, Kansas Agricultural Experiment Station, Manhattan.

² E. H. Herrick, Professor, Carlton Paulson and Robert Baron, Graduate Research Assistants, Zoology; C. B. Browning, Graduate Research Assistant, Dairy, now at Mississippi State University.