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Preparation of Calcium-Sensitive -Casein

Eastern Regional Research Laboratory,¹ Philadelphia, Pennsylvania

ABSTRACT

A relatively simple modification of the method for fractionating whole casein in urea solutions is described which gives a calcium-sensitive -casein. The yield is about 40% of total -casein. This casein and -paracasein precipitate to the same extent with CaCl₂; 10 mM CaCl₂ per liter at pH 7 is sufficient to precipitate. a 1% solution of each casein. The two caseins can, however, be distinguished by their initial rates of aggregation with lower concentrations of CaCl₂ (1.5 to 3.5 mM per liter). With low concentrations of CaCl₂, whole -casein aggregates to a greater extent than does calcium-sensitive -casein. The calcium-sensitive -casein appears to be homogeneous on electrophoresis at pH 8.5 and 2.3. Very little (less than 1%) of this casein is made soluble by the action of rennin.

¹ Eastern Utilization Research and Development Division, USDA.