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Metabolism of Bovine Semen. VI. Effect of Fructose and Arabinose on the Uptake and Metabolic Utilization of Glycerol-1-C¹⁴ by Bovine Spermatozoa^{1, 2,}

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

The addition of 1.25% fructose to a saline—1% glycerol diluent resulted in less glycerol-1-C¹⁴ utilization by twice-washed bull spermatozoa than when fructose was omitted. Arabinose reduced glycerol utilization slightly. Significant quantities of fructose and arabinose disappeared during incubation of spermatozoa under nitrogen for 3 hr. at 37° C. No lactic acid was produced from arabinose, whereas fructose utilization resulted in the production of large amounts of lactate. Glycerol reduced anaerobic glycolysis of bull spermatozoa. Less sugar was utilized when 1% glycerol was added to saline, saline-fructose, and saline-arabinose diluents than when glycerol was omitted. Glycerol addition resulted in greater lactic acid production in saline and saline-arabinose diluents. However, in saline-fructose diluent less lactate accumulated when glycerol was added.

¹ Authorized for publication on August 1, 1958 as paper No. 2280 in the journal series of The Pennsylvania Agricultural Experiment Station.

² Supported in part by grants from the Pennsylvania State Association of Artificial Breeding Cooperatives.

³ The data contained in this paper are part of a thesis submitted by the senior author to the Graduate School of The Pennsylvania State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy.