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Journal of Dairy Science Vol. 42 No. 1 83-88
© 1959 by American Dairy Science Association ®
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Metabolism of Bovine Semen. V. Effect of Various Diluents on the Uptake and Metabolic Utilization of Glycerol-1-C14 by Bovine Spermatozoa1, 2,

W. T. O'Dell3, J. O. Almquist and R. J. Flipse

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

The effect of various commonly used semen diluents on the metabolism of glycerol-1-C14 by bull spermatozoa has been studied. Glycerol-1-C14 uptake and utilization by twice-washed sperm during incubation under nitrogen for 3 hr. at 37° C. were significantly (P < 0.01) less in skimmilk, homogenized milk, and 1:4 egg yolk-citrate diluents than in physiological saline diluent. Sperm in milk diluents metabolized more glycerol than did those in egg yolk-citrate. The presence of utilizable substances in milk and egg yolk may have been responsible for sparing glycerol utilization. Radioactive glycerol was associated with the sedimentable egg yolk solids in a 1:4 egg yolk-citrate diluent. Radioactivity was not diminished by washing the solids as many as six times with physiological saline-glycerol solution before assay.

FOOTNOTES

1 Authorized for publication on August 1, 1958 as paper No. 2279 in the journal series of The Pennsylvania Agricultural Experiment Station.

2 Supported in part by grants from the Pennsylvania State Association of Artificial Breeding Cooperatives.

3 The data contained in this paper are part of a thesis submitted by the senior author to the Graduate School of The Pennsylvania State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy.






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Copyright © 1959 by the American Dairy Science Association ®.