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Journal of Dairy Science Vol. 42 No. 1 74-78
© 1959 by American Dairy Science Association ®
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Bovine Semen Metabolism. IV. Effect of Added Hormones and Amino Acids on Fructolysis 1

M. Freund2 and J. P. Mixner

Department of Dairy Science, New Jersey Agricultural Experiment Station, Sussex

ABSTRACT

A total of 46 semen samples was used to determine the effects of the addition of the hormones, epinephrine, insulin, thyroxine, and triiodothyronine, and of the sulfhydryl-containing amino acids, ergothioneine and cysteine, on fructolysis in bull semen. A control tube and three tubes with the selected levels of compound addition and semen were incubated at 37° C. in a 2.9% sodium citrate buffer, and fructose utilizations were determined at 60 and 120 min. Epinephrine addition (4–100 µg. per milliliter) resulted in an inhibition of fructolysis during the 0- to 60-min. period, with a return to the control level during the 60- to 120-min. period. There was no apparent effect of insulin (4–100 µg. per milliliter); thyroxine (0.04–1.00 µg. per milliliter); triiodothyronine (0.008–0.200 µg. per milliliter); ergothioneine (4–100 µg. per milliliter), or cysteine (4–100 µg. per milliliter) on fructose utilization.

FOOTNOTES

1 Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers University, the State University of New Jersey, Department of Dairy Science, New Brunswick. This work was supported in part by a grant-in-aid from the National Association of Artificial Breeders.

2 Present address: Department of Physiology and Pharmacology, New York Medical College, Flower and Fifth Avenue Hospitals, New York City.






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Copyright © 1959 by the American Dairy Science Association ®.