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Journal of Dairy Science Vol. 42 No. 1 67-73
© 1959 by American Dairy Science Association ®
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Bovine Semen Metabolism. III. Effect of Fructose Concentration, Fructose Per Sperm, and Seminal Plasma Factor on Fructolysis1

M. Freund2, J. P. Mixner and R. E. Mather

Department of Dairy Science, New Jersey Agricultural Experiment Station, Sussex

ABSTRACT

A 4 x 22 balanced incomplete block factorial design was used to determine the effect of added fructose (total of four levels), fructose per sperm, and seminal plasma factor on fructolysis. Increased fructose concentration in the medium markedly increased fructose utilization at 20, 60, and 120 min. of incubation at 37° C. Increased total fructose per sperm also resulted in greater fructose utilization. Regressions of fructose utilization on logarithm of initial fructose in the incubation fluid differed among ejaculates, possible due to bull differences. There was no apparent effect of any seminal plasma factor on fructolysis other than its fructose content. The fructolysis coefficient (Kv) was unaffected by any of the treatments in this study, which gave further support to the possible usefulness of this method for comparing the metabolic activity of semen samples.

FOOTNOTES

1 Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers University, the State University of New Jersey, Department of Dairy Science, New Brunswick. This work was supported in part by a grant-in-aid from the National Association of Artificial Breeders.

2 Present address: Department of Physiology and Pharmacology, New York Medical College, Flower and Fifth Avenue Hospitals, New York City.






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Copyright © 1959 by the American Dairy Science Association ®.