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Journal of Dairy Science Vol. 42 No. 1 28-38
© 1959 by American Dairy Science Association ®
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Standardization of the Harland-Ashworth Test for Whey Protein Nitrogen1

S. Kuramoto2, Robert Jenness, S. T. Coulter and R. P. Choi

Departments of Dairy Husbandry and Agricultural Biochemistry, University of Minnesota, St. Paul, and American Dry Milk Institute, Inc., Chicago, Illinois

ABSTRACT

A standard procedure for performing the Harland-Ashworth whey protein nitrogen determination is presented. In this method, standard curves relating turbidity to the whey protein nitrogen content are prepared by using appropriate mixtures of salt filtrates prepared from two lots of nonfat dry milk, one containing a low, and the other a high, level of undenatured whey protein. Collaborative results secured from 17 laboratories using this method on six samples of nonfat dry milk indicated sufficient precision to warrant the use of this method as a basis of specifications for nonfat dry milk.

FOOTNOTES

1 Paper No. 3975, Scientific Journal Series, Minnesota Agricultural Experiment Station.

2 Present address: Land O'Lakes Creameries, Inc., Minneapolis, Minnesota.






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Copyright © 1959 by the American Dairy Science Association ®.