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Journal of Dairy Science Vol. 42 No. 1 188-189
© 1959 by American Dairy Science Association ®
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Effect of Glycerol on Lactic Acid Production by Bull Spermatozoa Diluted in Heated Skimmilk and Egg Yolk-Citrate1, 2,

W. T. O'Dell, J. O. Almquist and R. J. Flipse

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

The addition of glycerol either to milk or to egg yolk diluent improves the livability of bull spermatozoa (1, 5, 7, 9, 10), and its addition to milk diluent prolongs fertility (2, 7, 11). However, the mode of action of glycerol is not known. Flipse and Almquist (6) found that lactic acid production by bull spermatozoa during 3 hr. at 37° C. was less in milk-glycine-glycerol than in milk alone; whereas, oxygen consumption was not markedly altered by the presence of glycine plus glycerol. They postulated that the reduced accumulation of lactic acid might account for the extended survival of sperm in milk-glycine-glycerol diluent.

An experiment was designed to compare lactic acid production by bull spermatozoa in heated fresh skimmilk and 1:4 egg yolk-citrate diluents, with no additives, with 10% glycerol by volume, with 1.25% fructose by weight, and with 1.25% fructose plus 10% glycerol. With all treatments whole, pooled semen was diluted to one-half the final, volume in diluent without additives and cooled to 5° C.

FOOTNOTES

1 Authorized for publication on June 11, 1958, as Paper No. 2265 in the journal series of The Pennsylvania Agricultural Experiment Station.

2 Supported in part by grants from the Pennsylvania State Association of Artificial Breeding Cooperatives.






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Copyright © 1959 by the American Dairy Science Association ®.