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Fertility of Bovine Semen Extended at 28–30° C. in Egg Yolk-Citrate with or without Glycerin¹

Kansas Artificial Breeding Service Unit, Department of Dairy Husbandry, Kansas State College, Manhattan

ABSTRACT

Several workers (1, 2, 3) have reported that glycerin added at 5° C. to partially extended semen prolongs the livability and fertility of the spermatozoa when the semen is stored at 5° C. To be practicable at this bull stud, this addition at 5° C. of glycerin to semen which is to be stored and used as a liquid, probably would have to be performed in a refrigerated room, a costly item. The experiments to be noted here were conducted to determine whether glycerination of semen at 28–30° C. would be effective in improving livability and fertility of spermatozoa.

At this laboratory, in April and May of 1957, semen livability trials were conducted to determine simultaneously not only the effects of glycerination at different temperatures but also the effects of two variations in the rate at which glycerin may be added when extending semen to a constant motile sperm count and glycerin content. Semen samples were divided into two equal portions.

¹ Contribution No. 264, Kansas Agricultural Experiment Station, Manhattan.