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Journal of Dairy Science Vol. 41 No. 8 1110-1112
© 1958 by American Dairy Science Association ®
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Spermatozoa Survival in Milk Diluent with and without Seminal Plasma1

J. L. Albright2, M. H. Ehlers and R. E. Erb

Department of Dairy Science, State College of Washington, Pullman

ABSTRACT

With the acceptance of heated, homogenized milk as a successful extender for semen (2, 4, 9, 12) there is need for additional information on the nutritive sources in milk for sperm. It has been reported that spermatozoa are not capable of utilizing disaccharides under strictly anaerobic conditions (10), but are able to use monosaccharides as an exogenous source of energy (3, 8, 13).

Since a disaccharide, lactose, is the primary carbohydrate in milk extender, it is of interest to learn whether sperm can use this energy source. Milk lactose is relatively stable and little, if any, glucose or galactose would be liberated upon heating in the absence of acid (6). It is possible to prepare milk extenders which satisfactorily maintain sperm motility without using heat treatment (7).

This experiment was conducted to determine whether sperm cells removed from seminal plasma could retain motility when placed in variable amounts of milk and 2.9% Na citrate extender.


FOOTNOTES

1 Scientific paper No. 1668, Washington Agricultural Experiment Station, Pullman. Project 1107.

2 Permanent address: California Polytechnic College, San Luis Obispo, California.







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Copyright © 1958 by the American Dairy Science Association ®.