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Estimation of Bull Sperm Concentration by Packed Cell Volume

Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

Estimation of sperm concentration in this laboratory by the photoelectric method has been shown to be more rapid and slightly more repeatable than by hemacytometer counts (1, 3, 6). A third procedure employing the packed cell volume, or hematocrit technique, used widely for determining corpuscular volume in blood, was investigated as a new method for estimating sperm concentration in semen. Subsequently, it was found that considerable exploratory work had been done by Shaffner and Andrews (4) and recently Hickman (2) published a note on the "Spermatocrit Method."

In the present investigation, the International Hemacrit Centrifuge was used to pack the sperm in capillary tubes 75 mm. long and of 1.25 to 1.50 mm. O.D. The tubes were filled by capillary action, or by gently aspirating the semen into the tube with the aid of rubber tubing fitted with a mouthpiece. The latter procedure was faster, and minimized the possibility of a disproportionately large amount of seminal plasma flowing into the capillary tube.