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Effect of Hay-Concentrate Ratio on Rumen Fill as Measured by an Antipyrine Dilution Technique¹

Departments of Dairy and Microbiology and Public Health, Michigan State University, East Lansing

ABSTRACT

The rumen contents of seven fistulated bovines were removed and weighed at from 2 to 24 hr. after feeding. These 477- to 1,310-lb. animals were fed from 1.3 to 3.1 lb. of air-dry material per 100 lb. body weight per day in one portion. Regression equations were developed for the total contents and water contained in the reticulo-rumen as a function of the per cent concentrate in the ration, time after feeding, and plane of nutrition. The per cent concentrate in the ration had a much larger effect on the amount of rumen contents than either time after feeding or plane of nutrition. Hay-concentrate ratios did not significantly affect dry matter concentrations in the rumen. The dry matter content of rumen ingesta decreased with time after feeding (P < 0.01). An antipyrine dilution method was developed for estimation of the water content of the reticulo-rumen. The regression equations were found to give a better estimate than the antipyrine dilution values.

¹ Published with the approval of the Director of the Michigan Agricultural Experiment Station as Journal Article No. 2120.