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Flow Dialysis as a Means of Preserving Bovine Semen at Room Temperature¹

Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

A flow dialysis technique has been developed and used to prolong the life of bull sperm at room temperatures. Samples of undiluted semen in dialysis sacs were suspended in constantly flowing media of egg yolk-citrate, of milk, and of blood products. Undiluted semen controls in test tubes at room temperature lived for about 24 hr.; whereas, portions of the same samples undiluted in dialysis sacs survived for a week, with a yolk-citrate media constantly passing the sacs. Survival was better with the yolk-citrate mixture in the flow dialysis system than with any of the other media tried. Sperm life was supported better at room temperature than at 5° C. in the flow dialysis system. Bubbling O₂ through yolk-citrate media decreased the survival time of sperm compared to no gassing, whereas N₂ improved survival. Results from gassing with CO₂ varied from much improvement in survival at times to reduced survival in others. Further studies on the effects of CO₂ on bull sperm are in progress.

¹ The data presented in this paper were taken from a thesis submitted by the junior author to the Graduate School of the University of Illinois in partial fulfillment of the requirements for the Master's degree.

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