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Motility of Bovine Spermatozoa Stored at 5° C. when Extended in Mixtures of Yolk-Citrate, Yolk-Glycine, Whole Milk, Skimmilk, and Glycerol^{1, 2,}

Department of Dairy Science, State College of Washington, Pullman

ABSTRACT

Four semen samples were collected from each of three bulls. Each semen sample was divided into a glycerolated (7.5%) and a nonglycerolated group. Each group was extended 1:24 into ten different extenders at an initial temperature of 32° C. The extenders were egg yolk-eitrate (YC), whole milk (WM), skimmilk (SM), and egg yolk-glycine (YG), and their combinations were made up in equal proportions. Progressive motility rated on a 0–10 scale (10 = 100%) was used to estimate the desirability of the extenders during storage at 5° C. YC, WM, SM, and YG were, on the average, lower in motility after extension at Day 3 and Day 7 of storage (P < 0.01) than their combinations. Both combinations, WMYG and SMYG, showed higher average motility on Day 7 (P < 0.01) than did WMYC, SMYC, WMSM, or YCYG. YCYG was superior (P < 0.01) to either YC or YG alone. WM, SM, and WMYC had higher motility after extension (P < 0.05) than the same extenders containing glycerol. No other comparisons involving glycerol showed statistically significant differences. Under the conditions of this study, 2.9% glycine enhanced the ability of sperm to maintain progressive motility under storage at 5° C. when used with WM, SM, or YC.

¹ Scientific Paper No. 1669, Washington Agricultural Experiment Station, Pullman. Project 1107.

² The data are from a thesis presented by the senior author to the graduate faculty of the State College of Washington in partial fulfillment of the requirements for the Ph.D. degree.

³ Present address: California State Polytechnic College, San Luis Obispo.