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Centrifugal Tube for Extracting Butyl Alcohol in the Phosphatase Test

Department of Food Technology, University of Illinois, Urbana

ABSTRACT

Culture tubes, 1.6 cm. by 15 cm., are used to develop color in the phosphatase test of Sanders and Sager.₁ The color is extracted with 5 ml. of butyl alcohol and the sample is centrifuged with special tubes that fit into the trunnion cups. Four milliliters of the extracted butyl alcohol is used for the photometric estimation of phenol. If more than 4 ml. is needed, it is suggested that large tubes be used and the color extracted with the necessary amount of butyl alcohol in both the test and standard. However, it was found that culture tubes for centrifugation were not satisfactory.

A modified Pyrex tube, which fits into the trunnion cup of a Babcock centrifuge 40.6 cm. in diameter, was designed. It is shown in Figure 1 with dimensions. The capacity of the bulb is 10–12 ml., which is about equal to the total volume of test solution and the reagents used to develop color.