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Effect of Incubation Vessel on the Recovery of C¹⁴O₂ during Incubation of Bull Spermatozoa with Glycerol-1-C^141,2,

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

A report by Salisbury and Sharma (6) showed that oxygen uptake of bull spermatoza diluted in 0.9% saline varied directly, but not proportionately, with the ratio of the exposed surface area-to-volume (S/V) of the fluid in the incubation vessel. Maximum oxygen uptake was obtained with an S/V ratio in the range of 1–1.9. A series of experiments investigating the uptake and metabolic utilization of glycerol-1-C¹⁴ by frozen and unfrozen bull spermatozoa has been conducted in this laboratory (4, 5). In the course of these experiments it was found that radioactive C¹⁴O₂ recovery during incubation of spermatozoa varied with the type of incubation vessel employed.

Therefore, an experiment was designed to investigate the following incubation techniques: (a) 125-ml. Erlenmeyer flasks vs. 20- by 180-mm. test tubes as incubation vessels, and (b) incubation at 5° C. for 18 hr. vs. 37° C. for 3 hr. The vessels were not agitated during incubation.

¹ Authorized for publication on June 15, 1958, as Paper No. 2269 in the journal series of the Pennsylvania Agricultural Experiment Station.

² Supported in part by grants from the Pennsylvania State Association of Artificial Breeding Cooperatives.

³ The data contained in this paper are part of a thesis submitted by the senior author to the Graduate School of The Pennsylvania State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy.