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Fertility of Bull Spermatozoa in Yolk-Citrate Partially Replaced by Glucose

American Foundation for Biological Research, Madison, Wisconsin

ABSTRACT

Four livability trials and three breeding trials were conducted to compare two diluters: (a) YGC—one part egg yolk, four parts of a 5% solution of glucose, and one part of a 2.9% solution of sodium citrate dihydrate, and (b) YC—one part egg yolk and one part of a 2.9% solution of sodium citrate dihydrate. The means of the progressive motilities for 50 ejaculates stored in these diluters for seven days at 5° C. were not significantly different (P > 0.05). After one day of storage, the difference between the average nonreturn rates for the two diluters was not significant (P > 0.05). There was a total of 6,405 first services. After two days of storage, with a total of 1,914 services, the 60- to 90-day nonreturn rate with YGC was 61.7, and with YC, 55.7. The difference is highly significant (P < 0.01). These data support published livability studies which have indicated that a high concentration of electrolytes is detrimental to spermatozoa.

¹ Present address: Dairy Department, Michigan State University, East Lansing.