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Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park
ABSTRACT
Comparisons of the glycolytic activity of bovine spermatozoa at 37° C. over a 3-hr. period have been made in heated milk (M), milk-glycine (MG), milk-glycerol (M-glycerol), and milk-glycine-glycerol (MGG), and in Ringer-phosphate (R), Ringer-phosphate-glycine (RG), and Ringer-phosphate-glycine-glycerol (RGG). Measurements of lactate revealed greater accumulation in M and in R than in the glycine and/or glycerol-containing diluents. Fructose changes were variable and showed no consistent trends.
When the oxygen uptake by washed spermatozoa was measured in the milk diluents, only slightly higher values were obtained in MG and MGG than in M, but when saline was used instead of milk, appreciable stimulation of oxygen uptake occurred in the glycine and/or glycerol-containing preparations.
1 Authorized for publication as paper No. 2264 in the journal series of the Pennsylvania Agricultural Experiment Station.
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