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Freeze-Drying Bovine Spermatozoa

State College of Washington, Pullman, Washington

ABSTRACT

Lyophilization is being used for preserving blood plasma, antibiotics, milk, bacteria, tissue culture media, viruses, and various metazoan cells. Neumann (4) found that live tumor cells would withstand freeze-drying. Passey and Dmochowski (5) had reported earlier that living tumor cells could be observed only after partial drying. The literature on freeze-drying of spermatozoa of all species is limited. Polge et al. (6), using a high-vacuum distillation process on fowl semen, recovered motile spermatozoa. Sherman (7) observed negative results with human spermatozoa, when using freeze-drying procedures commonly employed in preparation of materials for cytological studies.

A freeze-drying study was conducted using five bulls (three Holsteins, one Guernsey, and one Jersey). Eight samples were collected and extended 1:4 at 95° F. into whole milk containing 1,000 µg. of streptomycin/ml of extended semen. The extended semen was then placed in a 400-ml. beaker filled one-third with water at 85° F. and allowed to cool for 20 min. (to approximately 65° F.) at 40° F.