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Journal of Dairy Science Vol. 41 No. 1 190-202
© 1958 by American Dairy Science Association ®
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Anaerobic Purine Dissimilation by Washed Suspensions of Bovine Rumen Bacteria1,2,

P. Jurtshuk, Jr., R. N. Doetsch and J. C. Shaw

Departments of Microbiology and Dairy Husbandry, University of Maryland, College Park

ABSTRACT

Xanthine, uric acid, and guanine hydrochloride were degraded completely, at a slow rate, by WCS of bovine rumen bacteria. The end-products formed were carbon dioxide, ammonia, and acetic acid, and the fermentation pattern was similar to that observed in clostridial-type organisms. Hypoxanthine was degraded incompletely, whereas adenine was neither decarboxylated nor deaminated under the conditions employed. Gases other than carbon dioxide (hydrogen and/or methane) never were encountered as end-products of purine degradation and neither were lactic, formic, propionic, and butyric acids. With the exception of hypoxanthine, WCS deaminated purines to a greater extent under alkaline than under acidic conditions. In experiments dealing with xanthine decarboxylation, less carbon dioxide was formed from this substrate than that observed for the corresponding endogenous value. This was not true of other purines. Kinetic treatment of the data suggested that a carbon dioxide utilizing or fixing mechanism was involved in xanthine dissimilation. Purine interconversions could not be demonstrated by paper chromatography. Differential spectrophotometrie methods showed that xanthine and uric acid were degraded completely, hypoxanthine was partially degraded, and adenine was not dissimilated.

FOOTNOTES

1 Hereafter, WCS designates washed cell suspensions of bovine rumen bacteria.

2 Scientific Article No. A632, Contribution No. 2814 of the Maryland Agricultural Experiment Station.






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Copyright © 1958 by the American Dairy Science Association ®.