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Detection of 1-Monoglycerides in the Fat From Rancid Milk

Department of Animal Industries Storrs Agricultural Experiment Station, Storrs, Connecticut

ABSTRACT

Recent work indicates that pancreatic lipase hydrolyzes triglyeerides to 1,2-diglyeerides and then to 2-monoglycerides (1,2).The 1-monoglycerides isolated in these studies are believed to result from isomerization of the 2-monoglycerides (1). Assuming that milk Upases act in the same manner, these partial glycerides should be present in the fat of rancid milk.

Two samples (900 ml. and 2,700 ml.) of rancid milk, prepared by mixing raw and homogenized milk in equal portions, were extracted with Mojonnier reagents and the solvents evaporated. The fat thus obtained was partitioned between heptane and 80% ethanol, as described by Borgstrom (3). The l-monoglyceride contents were determined by the periodic acid oxidation method described by Martin (5) and are listed (Table 1). A factor of 298, based upon a butterfat saponification value of 236, was used in the calculation of the monoglyeeride content.

Attempts to increase further the l-monoglyceride content of the 80% ethanol fractions by chromatographic fractionation on silicic acid columns, with chloroform containing varying amounts of methanol, were not successful.