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Journal of Dairy Science Vol. 40 No. 9 1099-1106
© 1957 by American Dairy Science Association ®
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Factors Influencing Metabolic Activity of Bull Spermatozoa. II. Cold-Shock and its Prevention

A. W. Blackshaw1 and G. W. Salisbury

Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

Rapid cooling of diluted bull semen from 25 to near 0° C. (cold-shock) produced a marked loss of motility, respiratory activity, and glycolysis. The motility and metabolic damage produced by rapid cooling could be partially prevented by lecithin and almost completely so by egg yolk. The egg yolk also stimulated the glycolysis of unshocked semen. Cold-shock of whole semen caused a loss of potassium and ether alcohol-extractable phosphorus from the cells into the plasma, and an uptake of sodium and calcium by the cells.

FOOTNOTES

1 Present address: Department of Veterinary Physiology, University of Sydney, Sydney, Australia.






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Copyright © 1957 by the American Dairy Science Association ®.