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Department of Dairy Science, Ohio Agricultural Experiment Station, Wooster and The Ohio State University, Columbus
ABSTRACT
Twelve ejaculates from nine bulls were frozen and stored under mechanical and dry-ice refrigeration. Portions of each ejaculate remained under the two storage conditions for a 24-week period. At various intervals, semen samples were transferred from one storage condition to the other. Samples were evaluated at the end of each period for per cent of live cells and motility, to obtain an estimate of the quality of the sample.
Under conditions of the experiment, evaluations of the samples indicate that mechanical refrigeration is superior to dry-ice refrigeration in maintaining quality of frozen semen. It is also indicated that semen samples may be transferred from mechanical refrigeration (–96° C.) to dry ice, and vice versa, without reducing their quality below that normally obtained by dry-ice refrigeration.
1 Contribution to North Central Regional Project NC-2, Improvement of Dairy Cattle Through Breeding.
2 The material herein reported was taken from the data submitted by the senior author in partial 'fulfillment of the requirements for the degree, Master of Science, The Ohio State University, Columbus, Ohio, 1953.
3 The study was made possible through the assistance of Central Ohio Artificial Breeding Association, Richard Kellogg, Manager.
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