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Surface-Active Agents as Constituents of Diluents for Deep Freezing of Bovine Spermatozoa¹

Department of Dairy Husbandry, University of Wisconsin, Madison

ABSTRACT

Storage of semen at low temperatures encompasses two categories of spermatozoan physiology—snamely, survival and the retention of fertilizing capacity. Approximately 30 to 50 per cent of buil spermatozoa is immobilized by the freezing and thawing procedures now used (1, 4, 5, 8, 9, 10).

Lovelock and Rees (7) demonstrated that some detergents caused an increase, but others a decrease, in resistance of red blood cells to thermal shock. They attributed this to a modification in the phospholipid-cholesterol ratio of the cell surface as a result of action of the detergents. Koefoed-Johnsen and Mann (6) found that the addition of a surface-active agent would presumably alter the permeability of the sperm-cell membrane by changes brought about in the lipid sperm-cell capsule. The surface-active agents also lowered fructolysis and respiration.

The purpose of this study was to ascertain if the addition of a surface-active agent to the glycerolated extender would significantly increase the survival of bull spermatozoa subjected to freezing and thawing.

¹ Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. This work was supported in part by a grant from Badger Breeders' Cooperative, Consolidated Breeders' Cooperative, East Central Breeders' Cooperative, Southern Wisconsin Breeders' Cooperative, and Tri-State Breeders' Cooperative. It is a contribution from the Wisconsin Agricultural Experiment Station, as a collaborator under the North Central Region Cooperative Research Project entitled, "Improvement of Dairy Cattle Through Breeding—NC-2", conducted in cooperation with the Dairy Husbandry Research Branch, USDA.

² Agents of the Dairy Husbandry Research Branch, USDA.