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The Millipore Filter for Enumerating Coliform Organisms in Milk¹

Department of Food Technology, Massachusetts Institute of Technology, Cambridge

ABSTRACT

The presence of viable coliform organisms may be used in the dairy industry as an index of improper processing, inadequately cleaned or sterilized equipment, or careless handling of pasteurized milk. Although the presence of these organisms does not necessarily indicate a health hazard, it should be regarded as an indication of potential danger.

In all procedures suggested in the tenth edition of the Standard Methods for the Analysis of Dairy Products, by which coliform organisms in milk may be enumerated, it is difficult, if not impossible, to analyze a ten-ml. sample of milk at one time. If ten ml. of milk is to be tested, one ml. is plated in each of ten plates, or 3.3 ml. in each of 3 plates, the coliform count per ten ml. being the total number of coliform colonies on all plates.

The Millipore Filter (MF) technique has become well known in the field of bacteriology, particularly in water bacteriology.

¹ Contribution No. 313 from the Department of Food Technology. Based upon a paper presented at the 35th Annual Meeting of the Vermont Dairy Plant Operators' and Managers' Association, October, 1956.