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Fertility Results with Frozen Semen Stored up to One Year¹

New Jersey Agricultural Experiment Station and Sussex County Cooperative Breeding Association, Inc., Sussex

ABSTRACT

Five split ejaculates of semen from five Holstein bulls were used to determine the effects of freezing and the length of storage of frozen semen at –79° C. on the fertility of the semen. The semen was frozen in a diluter composed of egg yolk, sodium citrate, glycerol, streptomycin, and water. The mean fertility of the fresh control semen (175 cows) was 68.0%. For the frozen semen after storage at –79° C. for 7 to 14 days (99 cows), 6 months (97 cows), and 12 months (102 cows), the mean fertility was 66.7%, 70.1%, and 65.7%, respectively, as measured by the number of first-service cows not returned for service 60 to 90 days after insemination.

¹ Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers University, the State University of New Jersey, Department of Dairy Industry, New Brunswick.

² Manager, Sussex County Cooperative Breeding Association, Inc., Sussex, New Jersey.