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Journal of Dairy Science Vol. 40 No. 4 351-355
© 1957 by American Dairy Science Association ®
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Some Factors Affecting the Determination of Plasma Protein-Bound Iodine, Using the Alkaline Fusion-Ceric Sulfate Method1

H. D. Lennon, Jr. and J. P. Mixner

New Jersey Agricultural Experiment Station, Sussex

ABSTRACT

The determination of plasma protein-bound iodine (PBI) by the alkaline fusion-ceric sulfate method (2, 3) has been proposed as a simplification of older iodine distillation procedures (1, 4, 7). Few data have been presented that show the sources of experimental error in the use of the alkaline fusion method. This paper presents a study of the effect of the length and temperature of storage of bovine plasma on plasma PBI values, together with an analysis of error term variance as contributed by fusions and by replicate determinations within fusions. Also presented is a method of calculating plasma PBI values, whereby per cent transmittance readings, iodine standards, and simple proportionality calculations may be used.

METHODS AND PROCEDURES

Blood for this study was collected from the jugular vein of each of four dairy cows (three Holsteins and one Guernsey) which were of similar age and stage of lactation. The blood was centrifuged immediately to obtain the plasma.

FOOTNOTES

1 Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers University, the State University of New Jersey, Department of Dairy Industry, New Brunswick. This research was supported in part by Research and Marketing Funds (Sec. 10b) of the Agricultural Research Service, Dairy Husbandry Research Branch, U. S. Department of Agriculture.






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Copyright © 1957 by the American Dairy Science Association ®.