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Activation of Bovine Spermatozoa by the Use of Sodium Carbonate^{1, 2, 3,}

Department of Dairy Science, The Ohio State University, Columbus, and Ohio Agricultural Experiment Station, Wooster

ABSTRACT

Environmental factors affect the livability of semen. Better results were obtained by storing semen at 37° to 39° C. at pH 7.1 to 7.4 than at pH 6.5 to 6.8. However, at 4° to 6° C, the lower pH values gave the best results. The semen was activated wfth sodium carbonate. Editor.

Although good conception has resulted from procedures now in effect in artificial breeding, there is some possibility that even better results might be obtained if more information were available concerning the regulation of spermatozoan motility and its relation to conception. In general, two approaches have been used to study this problem: (a) the influence of various levels of pH, and (b) the effects of added stimulants.

Freshly collected semen usually has a pH of approximately 6.8, and most common diluents are buffered accordingly. Somewhat greater sperm activity may be observed, however, for short periods of time if the pH is increased.

¹ Contribution to North Central Regional Project NC-2, Improvement of Dairy Cattle Through Breeding.

² The material herein reported was taken from data submitted by the senior author in partial fulfillment of the requirements for the degree of Master of Science, The Ohio State University. 1953.

³ The study was made possible through the assistance of Central Ohio Breeding Association, Richard Kellogg, Manager.