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Fertility of Bovine Spermatozoa Stored at Minus 79° C. for One Week and for Seventeen Weeks

Department of Animal Husbandry, Cornell University, Ithaca, N. Y.

H. O. Dunn

New York Artificial Breeders' Cooperative, Inc., Ithaca, N. Y.

ABSTRACT

In a split-ejaculate experiment involving 75 ejaculates from eight Holstein sires during two 3-month experimental periods, the 60- to 90-day percentage nonreturns was 71.0 for 1,278 first services to unfrozen semen, 73.2 for 1,151 first services to frozen semen stored 1 week at –79° C., and 69.8 for 1,094 first services to frozen semen stored 17 weeks at –79° C. These differences, which were not significant even at the 10% level of probability, confirm the previous results with frozen semen reported from this laboratory. The average spermatozoan survival rate for all ejaculates was 77% after 1 week storage and 62% after 17 weeks storage at –79° C. The estimated number of motile spermatozoa per milliliter of extended semen at the time of breeding was 8.6 x 10⁶, 11.6 x 10⁶, and 9.3 x 10⁶ for the unfrozen, 1-week frozen, and 17-week frozen semen, respectively. Within treatments, there were no significant and useful correlations between the number of spermatozoa inseminated and fertility. A frozen semen kit capable of maintaining a temperature of approximately –79° C. for 6 days with a loss of 2 lb. of Dry Ice per day is described.

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