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Fertility Results with Frozen Semen Stored for Two Years¹

New Jersey Agricultural Experiment Station and Sussex County Cooperative Breeding Association, Inc., Sussex

ABSTRACT

Mixner and Wiggin² reported that in a split-ejaculate study, frozen semen from five Holstein bulls showed no significant decline in ertilizing capacity when stored for from 7 to 14 days, 6 mo., and 1 yr. at — 79° C., as compared to the unfrozen control semen. The semen was frozen in an egg yolk, sodium citrate, glycerol diluter containing streptomycin. The frozen semen bank referred to in that report has now been sampled after 2 yr. of storage at —79° C., to determine again its fertilizing capacity. Frozen semen from each of the five bulls was bred to approximately 20 first-service cows for a total of 102 breedings. The mean fertility rate at this time for the five bulls, based on 30- to 60-day nonreturns was 75.5%; whereas, the 60- to 90-day nonreturn rate was 65.7% (Table 1). This same semen used fresh and after storage at — 79° C. for from 7 to 14 days, 6 mo., and 1 yr. had 60- to 90-day nonreturn rates of 68.0, 66.7, 70.1, and 65.7%, respectively.

¹ Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers University, the State University of New Jersey, Department of Dairy Industry, New Brunswick.

³ Manager, Sussex County Cooperative Breeding Association, Inc., Sussex, New Jersey.