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Freezing Bovine Semen. I. Techniques for Freezing Bovine Spermatozoa in Milk Diluents^1,2,

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

Most of the research on freezing bovine spermatozoa has involved a combination of glycerol and egg yolk-citrate diluent, but several reports have appeared on frozen semen involving glycerol in combination with various milk diluents. Erickson et al. (5) and Mixner and Saroff (9) found 10% glycerol to be the optimum level for use in heated homogenized milk, whereas Jones et al. (7) recently reported that 7% was best. For heated skimmilk diluent, an early report from this station (11) indicated a requirement of from 10 to 13% glycerol, in contrast to reports of 7.5% by Mixner and Saroff (9) and 8% by O'Dell and Hurst (10).

With heated skimmilk, O'Dell and Almquist (11) found no significant differences in sperm survival after freezing among glycerol equilibration periods of 0.5, 4, and 18 hr. O'Dell and Hurst (10) compared glycerol equilibration times of 0.5 and 18 hr. for semen diluted in heated skimmilk and found a significant difference in favor of the shorter time.

¹ Authorized for publication February 25, 1957, as Paper No. 2130 in the Journal series of the Pennsylvania Agricultural Experiment Station.

² Supported in part by a grant from the Pennsylvania State Association of Artificial Breeding Cooperatives.