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Effect of Diphenyl-p-Phenylenediamine on the Utilization of Carotene and Vitamin A by the Calf^1,2,

Departments of Animal Husbandry and Chemistry, Iowa State College, Ames

ABSTRACT

Diphenyl-p-phenylenediamine (DPPD) has been found to reduce the loss of carotene in dehydrated alfalfa meal and, for a period of time, was used for this purpose in the feed industry. In chickens, it has been found to prevent encephalomalacia (5) and to improve the utilization of vitamin A (11) and of carotene (10), although in other research (3) DPPD was found ineffective with regard to the latter. DPPD failed to improve carotene absorption in an experiment with rats (7), although it was the only antioxidant studied which did not impair carotene absorption at the relatively high dietary concentrations employed. Carotene utilization was improved by inclusion of DPPD in the diet of cows on low carotene rations (17) and in the diet of calves fed alfalfa (14). The present study was carried out to determine the toxicity of DPPD and its effect upon the absorption of vitamin A and carotene from the gut of the dairy calf.

EXPERIMENTAL PROCEDURE

¹ Journal Paper No. J-3208, Iowa Agricultural Experiment Station, Ames. Project 1324.

² Research supported in part through funds provided by the B. F. Goodrich Chemical Company, Cleveland, Ohio.