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Rennet Action and Exchangeability of Calcium in Milk to Ion Exchange Resins

Dept. of Agricultural Chemistry, College of Agriculture, University of Missouri, Columbia

ABSTRACT

The exchangeability of calcium to ion exchange resins is known to be a specific property of milk, and variations in this, resulting from heat treatment and cool-aging, are measurable. These can be studied by the Resin-Contact-Time-Method (2). Thus, Baker and Gehrke (1) reported the influence of heat treatment on the exchangeable calcium in milk. Gehrke and Smith (3) have further studied the effects of heat and pH on the exchangeability of calcium and magnesium in milk. Rennet is known to influence the base-binding capacity of the casein (4), and it was thought of interest to see if rennet would cause any alteration in the nature and amount of exchangeable calcium in milk.

Using a buffered resin mixture consisting of 2.5 g. of K-form and 3.5 g. of H-form, of Amberlite IRC-50 in the dry state, which gave a pH of 6.8 on soaking in water overnight, the exchangeability of calcium was studied on fresh skimmilk, with and without added rennet.