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Livability of Spermatozoa in Diluters with Citrate and Bicarbonate Buffers and Yolk

American Foundation for Biological Research, Madison, Wisconsin

ABSTRACT

Three experiments on livability of spermatozoa are reported. The first demonstrated that livability of spermatozoa in yolk-glucose-bicarbonate is inferior to that in yolk-citrate, when spermatozoa are at a concentration of 15 x 10⁶ per milliliter. The second showed that there was no such difference with a dilution of 1:9, which gave an average of 180 x 10⁶ spermatozoa per milliliter. With the lower number of spermatozoa in yolk-glucose-bicarbonate, the pH remained above 7:00, but with a dilution level of 1:9, the pH after storage for ten days was 6.81. In the third experiment, survival of spermatozoa in yolk-glucose-bicarbonate with sulfanilamide and with 15 x 10⁶ spermatozoa per milliliter was equal to that in yolk-citrate.

It is hypothesized that metabolism of spermatozoa at the lower concentration in yolk-glucose-bicarbonate is stimulated by the high pH, but that sulfanilamide reduces or prevents this stimulation.