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Journal of Dairy Science Vol. 40 No. 10 1317-1323
© 1957 by American Dairy Science Association ®
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Effects of D-Limonene and {alpha}-D-Pinene on in Vitro Carbohydrate Dissimilation and Methane Formation by Rumen Bacteria

A. Crane1, W. O. Nelson and R. E. Brown

Laboratory of Bacteriology, Dept. of Dairy Science, University of Illinois, Urbana

ABSTRACT

9 x 10–4 M D-limonene, but not 9 x 10–4 M {alpha}-D-pinene, was toxic to stabilized rumen cultures which produce methane from acetic acid. Methane production from formic acid by rumen cultures was not affected by these terpenes.

Cellulose digestion, but not starch and cellobiose dissimilation by rumen bacteria, was markedly inhibited by higher concentrations of D-limonene or {alpha}-D-pinene.

FOOTNOTES

1 Present address: Department of Bacteriology, Indiana University, Bloomington.






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Copyright © 1957 by the American Dairy Science Association ®.