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Study of Some Chromogens in Cow Urine¹

New Jersey Agricultural Experiment Station, Sussex

ABSTRACT

Cow urine contains a group of chromogens which are apparently ionone derivatives of carotenoids of dietary origin. They are present in the conjugated state and may be hydrolyzed by either acids or B-glucuronidase. They are extractable with lipid solvents after hydrolysis. Specific absorption spectra were obtained with these chromogens by the use of the Zimmerman, Pincus, and Allen color reactions, but in each instance they differed from spectra obtained with 17-ketosteroids. These chromogens number at least six and react similarly to 17-ketosteroids on an alumina chromatographic column (benzene-ethanol system). The urinary excretion pattern of these chromogens displays a distinct diurnal rhythm. Studies to date on the measurement of 17-ketosteroids in cow urine have been confounded by the presence of these chromogens.

¹ Paper of the Journal Series, New Jersey Agricultural Experiment Station, Rutgers University — The State University of New Jersey, Department of Dairy Industry, New Brunswick. This research was supported in part by Research and Marketing Funds (sec. 10b) of the Agricultural Research Service, Dairy Husbandry Research Branch, U. S. Department of Agriculture.

² Address: Smith, Kline, and French Laboratories, Philadelphia.

³ Address: Dairy Department, Louisiana State University, Baton Rouge.