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Journal of Dairy Science Vol. 39 No. 8 1156-1160
© 1956 by American Dairy Science Association ®
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The Effect of Glycerol Equilibration Time on the Freezing of Bovine Spermatozoa in Egg Yolk-Sodium Citrate and Skimmilk Semen Extenders1,2

Glen D. O'Dell and Victor Hurst

Dairy Department, South Carolina Agricultural Experiment Station, Clemson

ABSTRACT

The time of the glycerol equilibration period is a matter of controversy among workers in the field of freezing bovine semen. The equilibration period may be defined as the time that sperm cells remain in a glycerolated extender prior to freezing.

Polge and Rowson (4) recommended an equilibration time ranging between 15 and 20 hours when using an egg yolk extender. Miller and VanDemark (2), comparing 2-, 6-, and 18-hour equilibration times when using an egg yolk extender, found the 6-hour period to be superior in terms of percentage of motile sperm cells surviving after freezing. Cragle et al. (1), also using an egg yolk extender, found 14.9 hours to be the most favorable equilibration time between 4 and 28 hours. Saroff and Mixner (5) showed a progressive increase in sperm cell survival in an egg yolk extender as the equilibration time was increased from 2 to 18 hours. O'Dell and Almquist (3), using a heated skimmilk extender, did not observe any significant difference in sperm cell survival after freezing when using 0.5-, 4-, and 18-hour equilibration times.

FOOTNOTES

1 Technical Contribution No. 246, South Carolina Agricultural Experiment Station.

2 Data in this publication are taken from a thesis submitted by the senior author to the Graduate School of the Clemson Agricultural College, Clemson, S. C. in partial fulfillment of the requirements for the M.S. degree.






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Copyright © 1956 by the American Dairy Science Association ®.