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Department of Dairy Husbandry, North Dakota Agricultural Experiment Station, Fargo
ABSTRACT
A method of deaerating milk samples by nitrogen ebullition is described. Twenty-five duplicate, individual cow milk samples have been deaerated by the method and lipase activity has been determined when fresh and after aging for 30 hours at 0° to 4° C. Twelve aged samples showed more, and 13 less, activity than their corresponding fresh samples. The mean activity of the fresh sampler was 3.940 µM of substrate hydrolyzed, and that of the aged samples, 3.901 µM. The difference (0.039 µM) was not statistically significant.
It is concluded that deaeration by nitrogen ebullition can serve as a useful means of preserving the lipase activity of fresh milk.
1 Published with the approval of the Director of the North Dakota Agricultural Experiment Station as a partial report on Bankhead Jones Project 39 "Estimation and Properties of Natural Milk Lipase(s)."
2 Present address: Washington State Agricultural Experiment Station, Pullman.
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