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Journal of Dairy Science Vol. 39 No. 5 568-573
© 1956 by American Dairy Science Association ®
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A Method for the Estimation of Peroxidase Activity in Milk1

L. W. Aurand, W. M. Roberts and J. T. Cardwell

Department of Animal Industry, North Carolina State College, Raleigh

ABSTRACT

The method which has been described possesses the advantages of speed, convenience, and sensitivity. In this method p-phenylenediamine is oxidized by lactoperoxidase in the presence of hydrogen peroxide. Under the conditions of the assay, the amount of color developed is proportional to time. The influence of hydrogen peroxide concentration, p-phenylenediamine concentration, and enzyme concentration was studied and the optimum conditions were selected.


FOOTNOTES

1 Published with the approval of the Director of Research, North Carolina Agricultural Experiment Station, as Paper No. 677 of the Journal Series.







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Copyright © 1956 by the American Dairy Science Association ®.