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Fluorimetric Estimation of Estrogens in Bovine Urine^{1, 2,}

Department of Dairy Science and Department of Veterinary Physiology and Pharmacology, State College of Washington, Pullman

ABSTRACT

A chemical method which is capable of extracting and estimating estrogen in the urine of cattle has been described. When applied to the urine of pregnant cattle, the total fluorescence given by this method increased with advance in pregnancy. The extent to which estrogenic compounds contributed to this variation has not been accurately determined over the whole range of values obtained. Bioassay values on a limited number of samples indicated that about one-fourth of the fluorescence was due to biologically active estrogens.

Further work on extraction and purification of the urine extracts and identification of the compounds contributing to the fluorescence is necessary to develop a satisfactory method for estrogens in cow urine.

¹ Scientific Paper No. 1391, Washington Agricultural Experiment Stations, Pullman. Project 1105.

² The data are from a thesis presented by the senior author to the graduate faculty of the State College of Washington in partial fulfillment of the requirements for the Ph.D. degree.

³ Present address: Department of Dairy Industry, Montana State College, Bozeman.

⁴ Present address: School of Veterinary Medicine, University of Minnesota, St. Paul.