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Yields and Protein Content of Holocellulose Prepared from Pepsin-Treated Forages

Dairy Husbandry Research Branch, USDA, Beltsville, Maryland

ABSTRACT

Retention of a considerable portion of the plant nitrogen in holocellulose preparations from forage plants has previously been reported (1, 3, 5). Since many forage plants contain considerable amounts of nitrogen, particularly in the early growth stages, it may be an important contaminant of holocellulose prepared from these materials. Acid chlorite treatment (chlorine dioxide) is usually considered as primarily a delignification process and has not been proposed for the preparation of holocellulose from forages containing considerable amounts of protein.

The object of this study was to ascertain the effect of pepsin incubation on the yields and nitrogen content of holocellulose prepared from various forages by acid chlorite treatment.

EXPERIMENTAL PROCEDURE

Ten forages ranging in protein (N x 6.25) content from 3.2 to 22.0% were used in this study. Nitrogen was determined by the conventional Kjeldahl procedure (2). Protein was calculated on the basis that it contained 16% of nitrogen.