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Diluters for Bovine Semen. IX. Motility of Bovine Spermatozoa in Milk-Glycine and Egg Yolk-Glycine Diluents with and without Glycerol¹

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

The motility and fertilizing capacity of sea urChin spermatozoa are beneficially affected by the inclusion of glycine in the diluting fluid (10, 11). Although attempts to improve bovine semen diluents by glycine addition have been made (5, 12, 13), only recently have such diluents shown promise of improving the livability of bovine spermatozoa during storage (2, 6).

The studies reported in this paper were undertaken to investigate the feasibility of using combinations of glycine and skimmilk as a diluent for bovine semen. In much of this work, nonfat dry milk solids was employed in an effort to develop a diluent that could be stored in dry form for long periods and that would support satisfactory spermatozoan livability and fertility after reconstitution.

EXPERIMENTAL PROCEDURE

The Instant Pet nonfat dry milk solids (NFDMS) used in these trials was purchased from local markets. Reconstitution was at the rate of 8.7 g. of NFDMS per 100 ml. of water.

¹ Authorized for publication on April 11, 1956 as paper No. 2054 in the journal series of the Pennsylvania Agricultural Experiment Station.