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An Agar Culture Medium for Lactic Acid Streptococci and Lactobacilli

Oregon Agricultural Experiment Station, Corvallis

ABSTRACT

In studies involving isolation of lactic streptococci from various sources it was observed that many of the agar media proposed for streptococci and lactobacilli did not support growth of fastidious strains of these organisms. Some media that did support growth had disadvantages from standpoint of ease of preparation or clarity of medium. Consequently a wider investigation was conducted primarily to evaluate additional agar culture media for their ability to support colony growth of fastidious lactic streptococci, particularly Streptococcus cremoris and S. lactis. Other streptococci and lactobacilli commonly employed as starter cultures in dairy products also were included in the study.

Lactic acid bacteria employed as test organisms in various media included: S. lactis strains E, 262, and ATCC 7963; S. cremoris 12, 101, 127, 144, 169, 208, 275, 300, 306, and ATCC 9596; S. cremoris var. aromaticus 4, 11, 20, and 22E; S. thermophilus S, C-3, and Mc; S. durans A; Lactobacillus acidophilus Farr; L. bulgaricus Gere A; L. lactis 39A; L. casei ATCC 7469; Leuconostoc dextranicum ATCC 8358 and 8086; and Leuc. citrovorum 1, 2, and ATCC 8081 and 8082.

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