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Some Carbonyl Compounds in Raw Milk¹

Ohio State University

ABSTRACT

The presence of alpha-ketoglutaric acid in pasteurized milk has been reported recently by Patton and Potter (3). It appears timely, therefore, to report the results of similar experiments completed at the time of publication of Patton and Potter's observations. The results confirm and supplement their findings.

A survey project has been in progress to determine the kind and concentration of various carbonyl compounds in fresh milk. During November and December, 1955, milk was obtained from 20 individual cows of the University herd. All cows were healthy, showing no clinical indication of either ketosis or mastitis. Fifty-ml. samples were obtained at the time of milking and were cooled to 4° C. The samples were immediately deproteinized by adding 5 ml. of 10% sodium tungstate, agitating for a few minutes, and then acidifying to pH 4 with 0.67 N sulfuric acid. The solid material was removed by centrifugation. The supernatant was filtered by suction through a Whatman No. 1 filter paper, dampened and dusted with washed silicic acid.

¹ Technical Note 6:56, Department of Dairy Technology, The Ohio State University and the Ohio Agricultural Experiment Station. O.A.E.S. Journal Article No. 63-56. 1956.