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Journal of Dairy Science Vol. 39 No. 11 1532-1541
© 1956 by American Dairy Science Association ®
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Identification of Constituent Amino Acids in a Peptide Stimulatory for Lactic Acid Bacteria1

W. E. Sandine2, M. L. Speck and L. W. Aurand

Department of Animal Industry, N. C. State College, Raleigh

ABSTRACT

Pancreas tissue was shown to contain two stimulants for L. casei and S. lactis which were active in the presence of strepogenin. One stimulant was purified chromatographically and showed characteristics of a peptide. Acid and alkaline hydrolyzates of the stimulant contained the following amino acids: lysine, aspartic acid, serine, glycine, glutamic acid, threonine, alanine, proline, valine, and probably both leucine and isoleucine. The relationship between the active stimulants in pancreas tissue and strepogenin was discussed.


FOOTNOTES

1 Published with the approval of the Director of Research, North Carolina Agricultural Experiment Station, Raleigh, as Paper No. 734 of the Journal Series.

2 Dairy Industries Supply Association Fellow.







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