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Dairy Breeding Research Center, The Pennsylvania State University, University Park
ABSTRACT
Earlier studies at this laboratory (1, 2, 15) and others (6, 7, 12, 16) have shown that fresh homogenized milk and skimmilk can be used as satisfactory diluents for bovine semen provided the milk is heated and cooled prior to semen dilution. However, Bolton and Durrell (3) found heated skimmilk to be inferior to egg yolk-citrate. Varying results have been reported by several artificial breeding organizations (14). A review of the latter reports indicated that a wide range of heating procedures was employed in the preparation of the milk diluents.
Michajilov (12) suggested that milk should be brought to a boil before it is cooled, whereas Thacker and Almquist (15) found that milk heated at 95° C. for 1 to 10 minutes supported optimum spermatozoan livability.
The objective of the present laboratory study was to determine the effect of the time and temperature of heating skimmilk upon its ability to support spermatozoan livability.
1 Authorized for publication on June 15, 1955, as Paper No. 1989 in the journal series of the Pennsylvania Agricultural Experiment Station.
2 The data contained in this paper are part of a thesis submitted by the senior author to the Graduate School of The Pennsylvania State University in partial fulfillment of the requirements for the degree of Master of Science.
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