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Yields of Holocellulose Prepared from Ruminant Feces by Acid Chlorite Treatment

Dairy Husbandry Research Branch, USDA, Beltsville, Md.

ABSTRACT

Holocellulose was prepared from the feces of cows fed ten different forages as their entire ration. A procedure was used that was satisfactory for the preparation of holocellulose from the ten forages fed. Five of the feces samples gave satisfactory recoveries of the calculated theoretical holocellulose contents and five gave higher than the calculated content. Repeated acid chlorite treatments caused a loss of carbohydrate from the holocellulose fraction with certain samples. Reducing properties of the holocellulose hydrolyzates indicate that a noncarbohydrate and nonreducing carbohydrate constituent is present in the holocellulose preparations which gave high yields. A uniform number of acid chlorite treatments with the conditions studied will not prepare holocellulose in theoretical yields with all samples from a variety of feces.