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A Multiple Regression Study of the Carr-Price Reaction for Vitamin A in the Presence of Carotene¹

Storrs (Conn.) Agricultural Experiment Station and the University of Connecticut

H. L. Lucas, Jr.

North Carolina State College

ABSTRACT

The use of the Carr-Price reaction for the estimation of vitamin A in the presence of interfering compounds, notably carotenoids, and the techniques employed to correct for such interferences have been amply reviewed (3, 5). In accord with good analytical procedure, methods which separate vitamin A and permit its estimation in the absence of interfering substances would appear to be preferable. For some biological materials such a separation is needed; however, in some instances, e.g., in bovine blood, - and ß-carotene (2) make up the principal interfering components, and the widely used correction of Dann and Evelyn (1) may be adequate. Where other interferences are operative, the use of internal standards is reported to allow the correct estimation of vitamin A (4).

Before attempting a determination of the magnitude and the nature of the interference in the Carr-Price reaction for bovine blood, it was deemed necessary to retest, with pure solutions, the validity of the method of correction used by Dann and Evelyn.

¹ This study was supported by funds provided by the Chas. M. Cox Company, Boston, Mass., and the Big-Y-Foundation, Norwich, Conn.